Thank you, I appreciate you taking the time to explain 😊

Do you do these extractions on the same sample, or do you have a sample for the ethanol, and another for the water? Lastly, does the sequence matter?

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Sorry, I didn’t see this question until just now! The extractions are done on the same sample, ethanol first followed by water. People do it both ways, but I think it’s better to do the ethanol first. Water can potentially oxidize and degrade some of the ethanol soluble compounds if you use water first.